CHARACTERIZATION AND N-TERMINAL SEQUENCING OF A CALCIUM-BINDING PROTEIN FROM THE CALCAREOUS CONCRETION ORGANIC MATRIX OF THE TERRESTRIAL CRUSTACEAN ORCHESTIA-CAVIMANA

Authors
LUQUET G TESTENIERE O GRAF F
Citation
G. Luquet et al., CHARACTERIZATION AND N-TERMINAL SEQUENCING OF A CALCIUM-BINDING PROTEIN FROM THE CALCAREOUS CONCRETION ORGANIC MATRIX OF THE TERRESTRIAL CRUSTACEAN ORCHESTIA-CAVIMANA, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1293(2), 1996, pp. 272-276
Citations number
31
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1293
Issue
2
Year of publication
1996
Pages
272 - 276
Database
ISI
SICI code
0167-4838(1996)1293:2<272:CANSOA>2.0.ZU;2-B
Abstract
We extracted proteins from the organic matrix of calcareous concretion s, which represents the calcium storage form in a terrestrial crustace an. Electrophoretic analyses of water-soluble organic-matrix proteinac eous components revealed 11 polypeptides, 6 of which are probably glyc osylated. Among the unglycosylated proteins, we characterized a 23 kDa polypeptide, with an isoelectric point of 5.5, which is able to bind calcium. Its N-terminal sequence is rich in acidic amino acids (essent ially aspartic acid). All these characteristics suggest its involvemen t in the calcium precipitation process within the successive layers of the organic matrix.