TESTING OF A DENGUE 2 LIVE-ATTENUATED VACCINE (STRAIN 16681 PDK 53) IN 10 AMERICAN VOLUNTEERS

A live-attenuated dengue 2 vaccine (strain 16681 PDK 53) developed at Mahidol University, Thailand was evaluated for safety and immunogenici ty by administering 10(4) p.f.u. subcutaneously to ten flavivirus non- immune American volunteers. The vaccine was safe, there were no seriou s adverse reactions. Eight recipients experienced no or mild side effe cts. One recipient reported headaches on 7 separate clays. One volunte er, who had a fracture of the humerus 1 day after vaccination requirin g surgical repair, experienced generalized malaise with fever (maximum temperature=38.9 degrees C), headache, eye pain and myalgia lasting l ess than 24 h. The vaccine was highly immunogenic, all recipients deve loped neutralizing antibody that persisted for two years. Copyright (C ) 1996 Published by Elsevier Science Ltd.