SEROLOGICAL CHARACTERIZATION OF ANTIENDOTOXIN SERA DIRECTED AGAINST THE CONJUGATES OF OLIGOSACCHARIDE CORE OF ESCHERICHIA-COLI TYPE R1, R2,R3, J5 AND SALMONELLA RA WITH TETANUS TOXOID

The covalent conjugates of oligosaccharide core: Escherichia coli type R1, R2, R3, J5 and Salmonella Ra with tetanus toroid have been prepar ed using reaction of reductive amination. The neoglycoconjugates were good immunogens in rabbits yielding a high level of anti-lipopolysacch aride antibodies of IgG class. The antibodies were used to examine the possibility of their reactions with smooth lipopolysaccharides. We ha ve found that all antisera were able to react with the lipopolysacchar ide molecules of identical or related core type, possessing core oligo saccharides substituted with O-specific chains. These reactions were s hown in both the ELISA assay and the immunoblotting test.