ANTIENDOTOXIN ANTIBODIES DIRECTED AGAINST ESCHERICHIA-COLI R-1 OLIGOSACCHARIDE CORE TETANUS TOXOID CONJUGATE BIND TO SMOOTH, LIVE BACTERIA AND SMOOTH LIPOPOLYSACCHARIDES AND ATTENUATE THEIR TUMOR-NECROSIS-FACTOR STIMULATING ACTIVITY

Authors
LUGOWSKI C NIEDZIELA T JACHYMEK W
Citation
C. Lugowski et al., ANTIENDOTOXIN ANTIBODIES DIRECTED AGAINST ESCHERICHIA-COLI R-1 OLIGOSACCHARIDE CORE TETANUS TOXOID CONJUGATE BIND TO SMOOTH, LIVE BACTERIA AND SMOOTH LIPOPOLYSACCHARIDES AND ATTENUATE THEIR TUMOR-NECROSIS-FACTOR STIMULATING ACTIVITY, FEMS immunology and medical microbiology, 16(1), 1996, pp. 31-38
Citations number
14
Categorie Soggetti
Immunology,Microbiology
Journal title
FEMS immunology and medical microbiology → ACNP
ISSN journal
09288244
Volume
16
Issue
1
Year of publication
1996
Pages
31 - 38
Database
ISI
SICI code
0928-8244(1996)16:1<31:AADAER>2.0.ZU;2-E
Abstract
The reactivity of anti-OS R1-tetanus toroid serum with various smooth and rough lipopolysaccharides have been determined in enzyme-linked im munosorbent assay (ELISA) and with intact, live smooth bacterial cells in flow cytometry. All tested smooth lipopolysaccharides of R1 core t ype belonging to nine different O-serotypes reacted strongly with anti -conjugate antibodies. Flow cytometry showed that anti-core antibodies labelled more than 95% of bacterial cells with high intensity of fluo rescence. The anti-conjugate serum was able to react with free form of smooth lipopolysaccharides and to inhibit their TNF stimulation activ ity in in vitro and in vivo models.