FINITE-SIZED COHERENT AND INCOHERENT DETECTORS IN CONFOCAL MICROSCOPY

Authors
Citation
T. Wilson et Jb. Tan, FINITE-SIZED COHERENT AND INCOHERENT DETECTORS IN CONFOCAL MICROSCOPY, Journal of Microscopy, 182, 1996, pp. 61-66
Citations number
12
Categorie Soggetti
Microscopy
Journal title
ISSN journal
00222720
Volume
182
Year of publication
1996
Part
1
Pages
61 - 66
Database
ISI
SICI code
0022-2720(1996)182:<61:FCAIDI>2.0.ZU;2-I
Abstract
We consider the effects of finite sized coherent and incoherent detect ors on the axial resolution of confocal microscopes. We adopt a high-a ngle vector approach which takes the polarization property of the obje ct into account. We further consider polarization imaging and show tha t coherent detection has an advantage over incoherent detection in ter ms of the value of the extinction coefficient. The desirability of abe rration correction is briefly discussed.