A COMBINED POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ENZYME ASSAY FOR DISCRIMINATING BETWEEN CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI

Authors
COMI G PIPAN C BOTTA G COCOLIN L CANTONI C MANZANO M
Citation
G. Comi et al., A COMBINED POLYMERASE CHAIN-REACTION AND RESTRICTION-ENDONUCLEASE ENZYME ASSAY FOR DISCRIMINATING BETWEEN CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI, FEMS immunology and medical microbiology, 16(1), 1996, pp. 45-49
Citations number
20
Categorie Soggetti
Immunology,Microbiology
Journal title
FEMS immunology and medical microbiology → ACNP
ISSN journal
09288244
Volume
16
Issue
1
Year of publication
1996
Pages
45 - 49
Database
ISI
SICI code
0928-8244(1996)16:1<45:ACPCAR>2.0.ZU;2-O
Abstract
A combined polymerase chain reaction and restriction endonuclease (RE) enzyme assay was developed to discriminate between Campylobacter coli and Campylobacter jejuni. Amplimers of the FlaA gene obtained by PCR were digested with AluI and HinfI to distinguish C, coli from C. jejun i. With AluI digestion C. jejuni-specific bands were observed at 110, 140 and 160 bp and C. coli-specific bands at 293 and 147 bp. C. jejuni -specific bands of 349 and 109 bp were found by HinfI digestion but Hi nfI did not digest the FlaA amplimer of C. coli. This combined techniq ue is fast and easy to perform, and distinguishes the two campylobacte rs unequivocally.