CONTINUOUS SEPARATION OF PROTEINS BY POLY(VINYL ALCOHOL) SHIELDED MULTICHANNEL FLOW ELECTROPHORESIS

Authors
LIU Z WANG J HUANG Z DING FX SHEN ZY YUAN NJ
Citation
Z. Liu et al., CONTINUOUS SEPARATION OF PROTEINS BY POLY(VINYL ALCOHOL) SHIELDED MULTICHANNEL FLOW ELECTROPHORESIS, Biotechnology techniques, 10(4), 1996, pp. 253-256
Citations number
4
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
4
Year of publication
1996
Pages
253 - 256
Database
ISI
SICI code
0951-208X(1996)10:4<253:CSOPBP>2.0.ZU;2-B
Abstract
A hydrophilic polysulphone membrane spaced multicompartment electrolyz er for Multichannel Flow Electrophoresis (MFE) was developed. Applicat ion of poly(vinyl alcohol) (PVA) in MFE resulted in a 35% increase of protein transmembrane flux. Continuous separation of bovine serum albu min (BSA) and haemoglobin bovine blood (HBB) model mixture by PVA shie lded MFE yielded 56mg BSA and 48mg HBB per hour. The average recovery was 65%.