NOVEL METHOD USING ANTIBIOTICS FOR THE DETERMINATION OF THE RATE-LIMITING STEP IN THE SECRETION PATHWAY OF GLUCOAMYLASE FROM RECOMBINANT YEAST

Authors
CHA HJ YOO YJ
Citation
Hj. Cha et Yj. Yoo, NOVEL METHOD USING ANTIBIOTICS FOR THE DETERMINATION OF THE RATE-LIMITING STEP IN THE SECRETION PATHWAY OF GLUCOAMYLASE FROM RECOMBINANT YEAST, Biotechnology techniques, 10(4), 1996, pp. 257-262
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
4
Year of publication
1996
Pages
257 - 262
Database
ISI
SICI code
0951-208X(1996)10:4<257:NMUAFT>2.0.ZU;2-0
Abstract
A novel method for the determination of the rate-limiting step in the protein secretion pathway in recombinant strains of Saccharomyces cere visiae is proposed which used several antibiotics that specifically in hibit the processing enzymes in the secretion pathway. This novel meth od has advantages such as the relative simplicity of the experiments, non-requirement of secretion mutants, and absence of strain effects.