LIPID CHARACTERISTICS OF PHYTOPHTHORA-CRYPTOGEA

Lipid synthesis was studied in Phytophthora cryptogea grown on various media. Ammonium succinate was found to be the most favorable source o f nitrogen for growth and lipogenesis; the highest lipogenic activity and nonsaturation of lipids were observed with this nitrogen source. E levation of the phosphate concentration enhanced the accumulation of b iomass and increased the lipid pool. Cultivation at lower temperature resulted in greater nonsaturation of the lipids. The molecular structu re of eicosapolyenoic fatty acids was identified. The localization of fatty acids in the main lipid fractions was studied. The complex conta ining dihomo-gamma-linolenic, arachidonic, and cis-5,8,11,14,17-eicosa pentaenoic acids was found to be predominantly localized in the glyco- and phospholipid fractions. The greatest amount of lipids excreted fr om the cells was registered during the exponential phase of culture gr owth, with an identical composition of intracellular and extracellular fatty acids.