D. Nakajima et al., PRESENCE OF 1-HYDROXYPYRENE CONJUGATES IN WOODY PLANT-LEAVES AND SEASONAL-CHANGES IN THEIR CONCENTRATIONS, Environmental science & technology, 30(5), 1996, pp. 1675-1679
beta-O-Glucoside and beta-O-glucuronide conjugates of 1-hydroxypyrene
(OHPy) were found in the leaves of four kinds of woody plant; cherry,
maple, ginkgo, and camphor. The conjugates were quantified by measurin
g OHPy liberated from the water-soluble fraction of leaves treated wit
h beta-O-glucosidase and beta-O-glucuronidase. Concentrations of beta-
O-glucuronide and beta-O-glucoside ranged from 0 to 358 and from 0 to
129 pmol/g of fresh leaves, respectively, in monthly measurement over
a period of 1 year. The amounts of glucuronide conjugates were greater
than those of glucoside conjugates in most plants examined. Both conj
ugates were detected at the highest concentrations in summer to autumn
. For most plants, the total amounts of the conjugates were greater th
an that of free OHPy in the leaves in all seasons, and the differences
reached 30-fold in maple leaves in September. From these results, the
source of the OHPy conjugates in leaves and their implications are di
scussed.