URINE CLEANUP METHOD FOR DETERMINATION OF 6 ARSENIC SPECIES BY LC-AASINVOLVING MICROWAVE-ASSISTED OXIDATION AND HYDRIDE GENERATION

Authors
LOPEZGONZALVEZ M GOMEZ MM PALACIOS MA CAMARA C
Citation
M. Lopezgonzalvez et al., URINE CLEANUP METHOD FOR DETERMINATION OF 6 ARSENIC SPECIES BY LC-AASINVOLVING MICROWAVE-ASSISTED OXIDATION AND HYDRIDE GENERATION, Chromatographia, 43(9-10), 1996, pp. 507-512
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
43
Issue
9-10
Year of publication
1996
Pages
507 - 512
Database
ISI
SICI code
0009-5893(1996)43:9-10<507:UCMFDO>2.0.ZU;2-P
Abstract
An effective clean-up method based on the precipitation of most high m olecular mass inorganic salts and organic compounds in ethanol at -15 degrees C has been developed for the determination of arsenite, arsena te, monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (A sB) and arsenocholine (AsC) in urine. Analyte recovery was close to 10 0 %. The six species were separated on an anionic HPLC column and dete rmined by microwave-assisted oxidation-hydride generation-atomic absor ption spectrometry. The detection limits and relative standard deviati on of the whole procedure were within the 8-15 mu g L(-1) and 2.5-5.3 % ranges, respectively, which allows application to the analysis of ur ine of people exposed to arsenic.