Br. Herbert et al., INVESTIGATION OF WOOL PROTEIN HETEROGENEITY USING 2-DIMENSIONAL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS, Electrophoresis, 17(1), 1996, pp. 239-243
The heterogeneity of intermediate filament proteins (IFP) from wool ha
s been investigated using two-dimensional polyacrylamide gel electroph
oresis with immobilised pH gradients in the first dimension. The charg
e heterogeneity has been confirmed with some of the IFP separated as a
string of spots with similar molecular weight, but markedly different
in isoelectric point (pI). The molecular weight and pI distribution o
f the string of IFP changed after treatment with alkaline phosphatase,
indicating that some of the heterogeneity is caused by phosphorylatio
n.