Es. Mendonsa et al., AN ENZYME FROM MYROTHECIUM-VERRUCARIA THAT DEGRADES INSECT CUTICLES FOR BIOCONTROL OF AEDES-AEGYPTI MOSQUITIO, Biotechnology letters, 18(4), 1996, pp. 373-376
Myrothecium verrucaria produced a high activity of extracellular insec
t cuticle degrading enzymes, chitinases, proteinases and lipases. Both
first (I) instar and fourth (IV) instar larvae of a mosquito, Aedes a
egypti, a vector of yellow fever and dengue, were susceptible to crude
culture filtrate (100% mortality within 48 h at 170 mg/l). The supple
mentation of purified M. verrucaria endo-chitinase with commercial lip
ase decreased the lethal time (LT(50)) from 48 h to 24 h for I instar
and 120 h to 96 h for IV Instar. Our results of the larvicidal activit
y indicate that the cuticle degrading enzyme complex of M. verrucaria
has a good potential for the control of mosquitoes.