The objective was to determine the effect of time of day (diurnal vari
ation), interval after feeding, prolonged recumbency, moisture on the
scrotum, and ambient temperature on scrotal surface temperature (SST)
in bulls as measured by infrared thermography. There was no significan
t effect of diurnal Variation on SST. Within 30 min after the start of
feeding, SST at the bottom of the testes and average SST increased, a
nd slowly declined thereafter. In bulls that were lying down for at le
ast 1 h, SST remained relatively constant after rising at an ambient t
emperature of 15 degrees C, but increased after rising at ambient temp
eratures of 5 and 25 degrees C. Ambient temperature had a large effect
on bottom SST, a small effect on top SST and an intermediate effect o
n average SST. The SST gradient (difference between top and bottom SST
) was greatest at 5 degrees C and least at 25 degrees C. When ambient
temperatures changed rapidly, there was an apparent overcompensation i
n SST (measured 3 h after ambient temperature stabilisation), followed
by a normalisation of the temperature (measured 24 h after temperatur
e stabilisation). Moisture on the scrotum decreased SST and following
drying returned to normal in approximately 30 min. Therefore, for repr
esentative SST measurement, scrotal infrared thermography can be perfo
rmed at any time of the day, but should not be done within several hou
rs of feeding or until 1 h after rising, and the scrotum should be dry
. Measurement of SST can be conducted over a wide range of ambient tem
peratures, although abrupt changes in ambient temperature may result i
n artifacts due to overcompensation.