I. Alexandrov et al., TUMOR-ASSOCIATED ANTIGENS ON RAT SARCOMA-CELLS IDENTIFIED BY MONOCLONAL-ANTIBODIES, Eksperimental'naa onkologia, 18(1), 1996, pp. 43-50
Two hybridoma clones have been produced by hybridization of murine mye
loma cell line PAI and splenocytes from BALB/c mice immunized with the
cells from a transplantable sarcoma induced in rat by SR-RSV. The ant
ibody produced by hybridoma clo-ne 2C2 was of subclass IgG3 and recogn
ized a cell surface antigen of 52 kD. It only cross-reacted with the c
ells from SR-RSV-induced sarcoma in hamster, but not with the cells fr
om the chicken RSV-induced sarcoma, nor with a number of methylcholant
hrene sarcomas and various other tumours of viral or another etiology
developed in rat, mouse, hamster or chicken. The antibody produced by
hybridoma clone 5G2 was of subclass IgG2A and recognized an antigen of
28 kD which was located under the plasma membrane and particularly in
the cell protrusions and microvilli. Cross-reactions were found with
all the sarcoma cells tested indicating that this antigen might repres
ent a common sarcoma antigen of comparatively low molecular mass.