ENZYMATIC-SYNTHESIS OF 4-O-BETA-D-GALACTOPYRANOSYL-MORANOLINE AND 3-O-BETA-D-GALACTOPYRANOSYL-MORANOLINE BY USING BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS

Authors
KOJIMA M SETO T KYOTANI Y OGAWA H KITAZAWA S MORI K MARUO S OHGI T EZURE Y
Citation
M. Kojima et al., ENZYMATIC-SYNTHESIS OF 4-O-BETA-D-GALACTOPYRANOSYL-MORANOLINE AND 3-O-BETA-D-GALACTOPYRANOSYL-MORANOLINE BY USING BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS, Bioscience, biotechnology, and biochemistry, 60(4), 1996, pp. 694-696
Citations number
10
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
4
Year of publication
1996
Pages
694 - 696
Database
ISI
SICI code
0916-8451(1996)60:4<694:EO4A3>2.0.ZU;2-1
Abstract
A transgalactosylation reaction from lactose to moranoline (1-deoxynoj irimycin) was accomplished by using beta-galactosidase [EC 3.2.1.23] f rom Bacillus circulans. The enzyme formed 3-O-beta-D-galactopyranosyl- moranoline and 4-O-beta-D-galactopyransoyl-moranoline as major product s, together with 2-O-beta-D-galactopyranosyl-moranoline and 6-O-beta-D -galactopyranosyl-moranoline as minor ones.