CLEAVAGE SPECIFICITY OF COXSACKIEVIRUS 3C PROTEINASE FOR PEPTIDE SUBSTRATE

Authors
MIYASHITA K OKUNISHI J UTSUMI R KOMANO T TAMURA T SATOH N
Citation
K. Miyashita et al., CLEAVAGE SPECIFICITY OF COXSACKIEVIRUS 3C PROTEINASE FOR PEPTIDE SUBSTRATE, Bioscience, biotechnology, and biochemistry, 60(4), 1996, pp. 705-707
Citations number
11
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
4
Year of publication
1996
Pages
705 - 707
Database
ISI
SICI code
0916-8451(1996)60:4<705:CSOC3P>2.0.ZU;2-M
Abstract
The substrate requirements of coxsackievirus 3C proteinase (3C(pro)) w ere investigated on the C-terminal side of the scissile bond using C-t erminal truncated peptides of the substrate peptide Ac-EALFQGPPV. Not only the Gln-Gly bond of Ac-EALFQG-NH2 but also the C-terminal amide g roup of Ac-EALFQ-NH2 was hydrolyzed by 3C(pro), suggesting that the es sential residues for cleavage by coxsackievirus 3C(pro) would exist wi thin the N-terminal 5 residues.