CLONING AND SEQUENCING OF TREHALOSE BIOSYNTHESIS GENES FROM RHIZOBIUMSP M-11

Authors
MARUTA K HATTORI K NAKADA T KUBOTA M SUGIMOTO T KURIMOTO M
Citation
K. Maruta et al., CLONING AND SEQUENCING OF TREHALOSE BIOSYNTHESIS GENES FROM RHIZOBIUMSP M-11, Bioscience, biotechnology, and biochemistry, 60(4), 1996, pp. 717-720
Citations number
11
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
4
Year of publication
1996
Pages
717 - 720
Database
ISI
SICI code
0916-8451(1996)60:4<717:CASOTB>2.0.ZU;2-A
Abstract
The two genes encoding maltooligosyl trehalose synthase and maltooligo syl trehalose trehalohydrolase, which are related to biosynthesis of a lpha,alpha-trehalose, were cloned from Rhizobium sp. M-11. Sequence an alysis showed that the synthase gene composed of 2316 bp was connected with the hydrolase gene of 1788 bp by an overlap of one nucleotide. T he deduced amino acid sequences of both enzymes have several regions c ommon to amylolytic enzymes belonging to ''alpha-amylase family''.