DETERMINATION OF GLUTATHIONE AND RELATED AMINOTHIOLS IN MOUSE-TISSUESBY GAS-CHROMATOGRAPHY WITH FLAME PHOTOMETRIC DETECTION

Authors
TAKAGI K KATAOKA H MAKITA M
Citation
K. Takagi et al., DETERMINATION OF GLUTATHIONE AND RELATED AMINOTHIOLS IN MOUSE-TISSUESBY GAS-CHROMATOGRAPHY WITH FLAME PHOTOMETRIC DETECTION, Bioscience, biotechnology, and biochemistry, 60(4), 1996, pp. 729-731
Citations number
27
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
4
Year of publication
1996
Pages
729 - 731
Database
ISI
SICI code
0916-8451(1996)60:4<729:DOGARA>2.0.ZU;2-L
Abstract
A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and gamma-glutam ylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractio ns, glutathione and aminothiols in each fraction were converted into t heir N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.