DETERMINATION OF NEUTRAL AND POLAR LIPIDS BY THIN-LAYER CHROMATOGRAPHY WITH FLAME IONIZATION DETECTION

The separation and simultaneous determination of the most frequent cla sses of the neutral lipid fraction (hydrocarbons, sterol esters, fatty acids, triglycerides, sterols, diglycerides and monoglycerides) and o f the polar lipid fraction (ceramides, monogalactosyl diglycerides, ce rebrosides, digalactosyl diglycerides, phosphatidyl inositol, phosphat idyl ethanolamine, phosphatidyl serine, lysophosphatidyl ethanolamine, phosphatidyl choline and lysophosphatidyl choline) of plant and anima l oils was accomplished by using thin-layer chromatography on Chromaro ds, flame ionisation detection, and a multiple stage development techn ique. Data were transformed through an unweighted straight line regres sion of the logarithm of peak area ratios on the logarithm of the weig ht ratios. The determinations were reproducible and all statistical es timates were highly significant. The linear concentration range, slope and intercept of the calibration curve are reported for each compound .