CYSTINE-STRING PROTEINS - A CYCLE OF ACYLATION AND DEACYLATION

We used tunicamycin, an inhibitor of protein fatty acylation, to exami ne the possibility that there is a cycle of acylation and deacylation of cysteine string proteins at nerve terminals. Using both physiologic al and immunoblot approaches, we obtained no evidence for a cycle of a cylation and deacylation that affects these proteins. These data sugge st that this lipid modification of cysteine string proteins is relativ ely more stable than that observed for other nerve ending proteins, li ke SNAP-25.