EPITOPES OF BORDETELLA-PERTUSSIS LIPOPOLYSACCHARIDES AS POTENTIAL MARKERS FOR TYPING OF ISOLATES WITH MONOCLONAL-ANTIBODIES

Three hybridomas (P1P3, D7 and 60.5) producing monoclonal antibodies ( mAbs) against Bordetella pertussis lipopolysaccharide (LPS) were estab lished, All reacted with the LPO from a typical, vaccine strain of B. pertussis (1414), but not with that of a variant strain (A100), Two of these mAbs (P1P3 and 60.5) cross-reacted with a B. bronchiseptica LPS ; only one (P1P3) reacted with a B. parapertussis LPS, ELISA reactivit ies with intact LPSs, and defined partial structures covalently linked to bovine serum albumin, were compared, mAb 60.5 bound to the termina l region of a distal trisaccharide consisting of N-acetylated amino su gars, D7 reacted with a substructure which can be modified in the B. p arapertussis and B. bronchiseptica LPSs by addition of a polymeric O-c hain, P1P3 bound to a nonacetylated glucosamine substituted with L-gly cero-D-manno-heptose, present in the 'core' of the B. pertussis LPS. T hese mAbs may be useful for rapid typing of Bordetella in clinical iso lates.