R. Takano et al., STRUCTURE OF A WATER-SOLUBLE POLYSACCHARIDE SULFATE FROM THE RED SEAWEED JOCULATOR MAXIMUS MANZA, Botanica marina, 39(2), 1996, pp. 95-102
The main acidic polysaccharide, designated PS1, extracted from the red
seaweed Joculator maximus Manza, consisted of D-galactose, L-galactos
e, 2-O-methyl-L-galactose, D-xylose and sulfate (100:69:32:49:73 in mo
lar ratio). Analysis based on partial hydrolysis led to the identifica
tion of oligosaccharides indicating a repeating backbone of -->3)beta-
D-Gal(1-->4)alpha-L-Gal(1-->. Within this structure, the (1-->4)-linke
d L-galactose residue was shown by trideuteriomethylation to be partly
2-O-methylated. On the basis of results obtained from C-13-NMR spectr
um analysis, controlled Smith degradation, specific 6-O-desulfation an
d methylation analyses, all the xylose residue appeared to occur as a
single unit branching in the beta-configuration at O-6 of the D-galact
ose residue, while the sulfates were located at O-6 of the D-galactose
residue and O-2 or O-3 of the L-galactose residue.