DETERMINATION OF THE TIME-COURSE OF AN ENZYMATIC-REACTION BY H-1-NMR SPECTROSCOPY - HYDROXYNITRILE LYASE CATALYZED TRANSHYDROCYANATION

Authors
HICKEL A GRADNIG G GRIENGL H SCHALL M STERK H
Citation
A. Hickel et al., DETERMINATION OF THE TIME-COURSE OF AN ENZYMATIC-REACTION BY H-1-NMR SPECTROSCOPY - HYDROXYNITRILE LYASE CATALYZED TRANSHYDROCYANATION, SPECT ACT A, 52(1), 1996, pp. 93-96
Citations number
20
Categorie Soggetti
Spectroscopy
Journal title
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY → ACNP
ISSN journal
13861425
Volume
52
Issue
1
Year of publication
1996
Pages
93 - 96
Database
ISI
SICI code
1386-1425(1996)52:1<93:DOTTOA>2.0.ZU;2-3
Abstract
The time course of the enzyme catalysed transhydrocyanation of benzald ehyde to give (S)-mandelonitrile was investigated using a hydroxynitri le lyase from Hevea brasiliensis as catalyst and acetone cyanohydrin a s cyanide donor. Employing special techniques it was possible to apply H-1 NMR spectroscopy in aqueous medium to monitor the concentration c hanges of all substrates and products. By this technique strong eviden ce for inhibition of the enzyme at higher substrate concentrations was obtained.