The nematode Maturity Index (MI) has been proposed as a means of detec
ting disturbance in marine and terrestrial biotopes. However, few stud
ies have dealt with the effect of the environment on MI. Using data ta
ken from an extensive study of a polluted estuary, a number of variabl
es were investigated to determine their effect on the MI. Sediment med
ian particle diameter, nematode population density and the heavy metal
elements analysed had no effect on values of the MI. However, number
of species present in a sample, distance from pollution source and pro
portion of different nematode feeding groups, especially feeding group
s 1B and 2A, had significant effects on values of the MI. The Maturity
Index was found to be a robust index and in general agreement with va
lues derived from traditional indices.