DNA-POLYMERASES FROM A PARASITIC PROTOZOA LEISHMANIA-DONOVANI UR6 - EVIDENCE OF PRESENCE OF A NOVEL KIND OF DNA-POLYMERASE

DNA polymerases of Leishmania donovani have been isolated and purified . The cell extract has been chromatographed on a phosphocellulose colu mn that separated into three peaks. The activity peak I was further pu rified to homogeneity. The DNA polymerase is a 64 KDa polypeptide, res istant to N-ethylmaleimide and aphidicolin. It requires MnCl2 and a hi gh concentration of KCl (0.5 M) for maximal activity. It has both 3' t o 5' and 5' to 3' exonuclease activities that reside in the same polyp eptide. (C) 1996 Academic Press, Inc.