A NEW MICROPLATE RED-BLOOD-CELL MONOLAYER TECHNIQUE FOR SCREENING ANDIDENTIFYING RED-BLOOD-CELL ANTIBODIES

Authors
LLOPIS F CARBONELLUBEROS F PLANELLES MD MONTERO M PLASENCIA I CARRILLO C
Citation
F. Llopis et al., A NEW MICROPLATE RED-BLOOD-CELL MONOLAYER TECHNIQUE FOR SCREENING ANDIDENTIFYING RED-BLOOD-CELL ANTIBODIES, Vox sanguinis, 70(3), 1996, pp. 152-156
Citations number
7
Categorie Soggetti
Hematology
Journal title
Vox sanguinis → ACNP
ISSN journal
00429007
Volume
70
Issue
3
Year of publication
1996
Pages
152 - 156
Database
ISI
SICI code
0042-9007(1996)70:3<152:ANMRMT>2.0.ZU;2-3
Abstract
A new method has been developed to immobilize red blood cells in wells of microplates using a cell fixation buffer. This method has been emp loyed for detecting and identifying red blood cell antibodies with gre ater sensitivity than haemagglutination antiglobulin tests, without lo ss of specificity. This method decreases the amount of test erythrocyt es and anti-human globulin reagents employed per test, consequently lo wering the cost.