The effects of fermentation of cassava by Aspergillus niger B-1 beta-g
lucosidase on its cyanide and protein content, and the optimal conditi
ons for this enzyme's activity, were examined, This fermentation proce
ss reduced the cyanide content of cassava by 95% to 2 mg/kg, and incre
ased its total protein content by 50%, thereby improving its nutrition
al value, A significant decrease in cyanogenic glycosides was detected
after 3 days of fermentation. The optimal pH for A. niger beta-glucos
idase activity on the cyanogenic glycoside linamarin was determined to
be 3, the optimal temperature 55 degrees C, and its K-m 0.3 mM. The f
indings presented here will facilitate the development of an improved
method for detoxification of cassava and for enhancement of its nutrit
ional value.