MORE DETAILED CHARACTERIZATION OF SAME OF THE HL-60 KARYOTYPIC FEATURES BY FLUORESCENCE IN-SITU HYBRIDIZATION

We performed a focused chromosome analysis on the HL60 cell line by mu lticolor fluorescence in situ hybridization (FISH), using probes for t he unequivocal identification of specific chromosome regions and subre gions. The purpose of this karyotypic re-evaluation rr as to confirm a nd to characterize in more detail chromosome rearrangements already id entified by means of classic cytogenetic approaches and recurrently de tected from the initial establishment of the cell line. The observatio ns reported may help reassess the potential of the HL60 cell line in u nderstanding the molecular events underlying the non-random karyotype alterations associated with acute myeloid leukemias (AML).