CHROMOSOMAL ASSIGNMENT OF 7 GENES ON CANINE CHROMOSOMES BY FLUORESCENCE IN-SITU HYBRIDIZATION

Our group has developed more than 600 DNA markers to build a map of th e canine genome. Of these markers, 125 correspond to genes (anchor loc i). Here we report the first six autosomal genes assigned to canine ch romosomes by fluorescence in situ hybridization (FISH), using cosmid D NA: adenine phosphoribosyl transferase on Chromosome (Chr) 3; creatine kinase muscle type on Chr 4; pyruvate kinase liver and red blood cell type on Chr 2; and colony-stimulating factor-1 receptor, glucose tran sporter protein-2, and tumor protein p53 on Chr 5. These assignments a re based on the karytotype proposed by Stone and associates (Genome 34 , 407, 1991) using high-resolution techniques. In addition, we have as signed the Menkes gene to the X Chr of the dog.