CONSTRUCTION OF A CYTOGENETICALLY CHARACTERIZED PORCINE SOMATIC-CELL HYBRID PANEL AND ITS USE AS A MAPPING TOOL

A new panel of cytogenetically characterized pig-rodent somatic cell h ybrids was constructed and tested for twelve microsatellite markers wi th PCR. Cytogenetic characterization of hybrids was accomplished by fl uorescence painting and GTG-banding of metaphase chromosomes. The pane l consists of 15 independent pig-hamster and 6 independent pig-mouse c ell Lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2-14, 2-15, 3-9, 14-15, 14-16, and 16-17. The microsatellites tested were S0022, S0023, S0084, S0098, S0112, S0113, S0114, S0115, S0117, S0 118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and c orrelation. Eight microsatellites could be assigned to specific pig ch romosomes, confirming seven assignments based on linkage analysis.