A COMPARATIVE MAP OF THE PORCINE AND HUMAN GENOMES DEMONSTRATES ZOO-FISH AND GENE MAPPING-BASED CHROMOSOMAL HOMOLOGIES

ZOO-FISH with chromosome-specific DNA libraries (CSLs) from individual flow-sorted human chromosomes was applied on porcine metaphase chromo somes to establish segment homology between the pig and human karyotyp es. Forty-seven porcine chromosomal segments corresponding to all huma n chromosomes except the Y were delineated, resulting in a nearly comp lete coverage of the porcine karyotype. The syntenic segments detected were further confirmed by the gene mapping information available in t he two species. A map demarcating physical boundaries of human homolog ies on individual pig chromosomes is complemented with a detail survey of the physical and genetic linkage mapping data in the two species. The resultant map, thus, provides a comprehensive and updated comparat ive status of the human and porcine genomes.