PROTECTIVE IMMUNITY IN BALB C MICE AGAINST THE SIMIAN-VIRUS 40-INDUCED MKSA TUMOR RESULTING FROM INJECTION OF RECOMBINANT LARGE T-ANTIGEN -REQUIREMENT OF CD8(+) T-LYMPHOCYTES/
J. Zerrahn et al., PROTECTIVE IMMUNITY IN BALB C MICE AGAINST THE SIMIAN-VIRUS 40-INDUCED MKSA TUMOR RESULTING FROM INJECTION OF RECOMBINANT LARGE T-ANTIGEN -REQUIREMENT OF CD8(+) T-LYMPHOCYTES/, The Journal of immunology, 156(10), 1996, pp. 3919-3924
BALB/c mice are often considered ''low responders'' or even ''nonrespo
nders'' with regard to cytolytic CD8(+) T lymphocytes and SV40 large T
Ag (TAg). Large TAg and fragments thereof were produced by recombinan
t technology and injected into BALB/c mice that were subsequently chal
lenged by i.p. injection of syngeneic TAg-expressing mKSA tumor cells.
Two portions of the TAg were found to induce protective immunity, one
stretching from amino acid residues 1-272 and the other from amino ac
id residues 683-708. In mice thus protected, the spleens were virtuall
y free of cytotoxic T cells but CD8(+) T lymphocytes obtained from the
peritoneal cavity during rejection of the mKSA cells were directly ly
tic for TAg-expressing target cells. Depleting immune mice of CD4(+) o
r CD8(+) T lymphocytes by treatment with mAb abolished their ability t
o resist tumor development. We conclude that immunity against SV40 TAg
-expressing tumor cells in BALB/c mice is dependent on both CD4(+) and
CD8(+) T lymphocytes.