SAGO STARCH AS A BIOMASS SOURCE - RAW SAGO STARCH HYDROLYSIS BY COMMERCIAL ENZYMES

Raw sage starch and sage starch pretreated by heating at 60 degrees C for 2 hours in sodium acetate buffer (pH 3.5) were hydrolysed using co mmercial glucoamylase-AMG (EC 3.2.1.3), alpha-amylases-BAN, Fungamyl a nd Termamyl (EC 3.2.1.1), debranching amylase-Promozyme (EC 3.2.1.41), and their mixtures in sodium acetate buffer pH 5.0 at 35 degrees C. R aw sage starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretr eating the starch increased the extent and rate of hydrolysis. A stron g synergism between glucoamylase and alpha-amylase on the hydrolysis o f both untreated and pretreated sage starch was observed The hydrolysi s products were characterized by high-performance size-exclusion chrom atography (HPSEC). The total carbohydrate concentration of hydrolysed sage starch decreased but the amylose and amylopectin ratios in the re sidues remained the same. (C) 1996 Elsevier Science Ltd.