DEVELOPMENT OF RAPD MARKERS IN TRITORDEUM AND ADDITION LINES OF HORDEUM-CHILENSE IN TRITICUM-AESTIVUM

RAPD markers were developed for octoploid XTritordeum (amphiploid Hord eum chilense x Triticum aestivum) and its parents. Two bread wheats, t wo H. chilense accessions and the two tritordeums synthesized with the m were used. A total of 41 arbitrary decamer primers were tested, yiel ding 190 products that could be assigned to wheat, 185 to H. chilense and 108 that were nonspecific (present in wheal and barley). A total o f 44 products were specific to one H. chilense line and 33 to the othe r 16 of the former were located on the chromosomes using a set of H. c hilense in T. aestivum addition lines. The potential of RAPDs for deve loping addition lines or the detection of introgressions of H. chilens e in bread wheat is discussed.