PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF AN ANTI-HIV-1 PROTEASE ANTIBODY THAT INHIBITS ENZYME-ACTIVITY

Authors
LESCAR J STOURACOVA R RIOTTOT MM CHITARRA V BRYNDA J FABRY M HOREJSI M SEDLACEK J BENTLEY GA
Citation
J. Lescar et al., PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF AN ANTI-HIV-1 PROTEASE ANTIBODY THAT INHIBITS ENZYME-ACTIVITY, Protein science, 5(5), 1996, pp. 966-968
Citations number
11
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
5
Issue
5
Year of publication
1996
Pages
966 - 968
Database
ISI
SICI code
0961-8368(1996)5:5<966:PCSOAA>2.0.ZU;2-#
Abstract
F11.2.32, a monoclonal antibody directed against the HIV-1 protease, d isplays strong inhibitory effects toward the catalytic activity of the enzyme. The antibody cross-reacts with peptides 36-46 and 36-57 from the protease. Crystals of the Fab have been obtained both in the free state and as complexes formed with the protease peptide fragments, 36- 46 and 36-57. Diffraction data have been collected for the free and co mplexed forms of Fab F11.2.32 and preliminary models for the crystal s tructures were obtained by molecular replacement.