HPLC DETERMINATION OF MORPHINE-ONDANSETRON AND MEPERIDINE-ONDANSETRONMIXTURES IN 0.9-PERCENT SODIUM-CHLORIDE INJECTION

High performance liquid chromatography procedures have been developed for the assay of morphine-ondansetron and meperidine-ondansetron mixtu res in 0.9% sodium chloride injection. The separation and quantitation of morphine-ondansetron were performed on an underviatized silica col umn at ambient temperature using a mobile phase of 60:40 v/v 0.01 M mo nobasic potassium phosphate pH 4.0 - methanol at a now rate of 1.0 mL/ min with the detection set at 233 nm. The separation was achieved with in 20 min. Morphine and ondansetron were linear in the 134 - 536 and 6 8 - 271 mu g/mL, ranges, respectivly. Accuracy and precision were in t he range 0.04 - 4 and 0.2 - 1%, respectively, for the two analytes and the limits of detection for morphine and ondansetron were 210 and 110 ng/mL, respectively, based on a signal to noise ratio of 3 and a 20 m u L injection. The separation and quantitation of the meperidine-ondan setron mixture were also achieved on an underivatized silica column at ambient temperature using a mobile phase of 60:40 v/v 0.01 M monobasi c potassium phosphate pH 4.0 - methanol at a flow rate of 1.0 mL/min w ith detection of the two analytes at 254 nm. The separation was achiev ed within 20 min. Meperidine and ondansetron were linear in the 556 - 3331 and 89 - 536 mu g/mL ranges, respectively. Accuracy and precision were in the range 0.7 - 5.1 and 0.1 - 0.6%, respectively, for the two analytes and the limits of detection for meperidine and ondansetron w ere 1.73 mu g/mL and 47 ng/mL, respectively, based on a signal to nois e ratio of 3 and a 20 mu L injection.