THE IN-VITRO FORMATION OF RECOMBINANT TAU POLYMERS - EFFECT OF PHOSPHORYLATION AND GLYCATION

tau Isolated from paired helical filaments, aberrant structures that a ppear in Alzheimer disease (AD) patients' brains, show at least two po sttranslational modifications: phosphorylation (Grundke-Iqbal et al., 1986; Ihara et al., 1986) and glycation (Ledesma et al., 1994; Yan et al., 1994). To test whether these modifications could affect the capac ity of tau to self-aggregate, recombinant tau was phosphorylated and g lycated, and its capacity to form polymers analyzed. Our results indic ate that on phosphorylation and glycation, the capacity of tau to form aggregates increases, and that glycation of tau could stabilize the a ssembled polymers and could facilitate formation of bundles from these polymers.