EVALUATION OF THE CHROMATOGRAPHIC BEHAVIOR OF FLUOXETINE AND NORFLUOXETINE USING DIFFERENT CYCLODEXTRINS AS MOBILE-PHASE ADDITIVES AND FLUOROMETRIC DETECTION
S. Piperaki et M. Parissipoulou, EVALUATION OF THE CHROMATOGRAPHIC BEHAVIOR OF FLUOXETINE AND NORFLUOXETINE USING DIFFERENT CYCLODEXTRINS AS MOBILE-PHASE ADDITIVES AND FLUOROMETRIC DETECTION, Journal of liquid chromatography & related technologies, 19(9), 1996, pp. 1405-1421
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The principal goal of this work was to investigate the liquid chromato
graphic retention behaviour of fluoxetine and norfluoxetine using HPLC
with respect to mobile phase composition, pH, flow rate and the amoun
t of the native beta-cyclodextrin (beta-CD) or the beta-hydroxypropyl-
cyclodextrin (HP-beta-CD), added to the mobile phase. Further, it was
of interest to evaluate the effectiveness of beta-cD and HP-beta-CD to
enhance fluorescence detection of these compounds. Another purpose of
this study, was to calculate the formation constants of the inclusion
complexes (K-f) of fluoxetine and norfluoxetine within the HP-beta-CD
, in different mobile phase compositions. Based on these findings, the
log K-f values of these two compounds referred to pure aqueous mobile
phase (log K-fw), can be determined by extrapolation.