EVALUATION OF THE CHROMATOGRAPHIC BEHAVIOR OF FLUOXETINE AND NORFLUOXETINE USING DIFFERENT CYCLODEXTRINS AS MOBILE-PHASE ADDITIVES AND FLUOROMETRIC DETECTION

The principal goal of this work was to investigate the liquid chromato graphic retention behaviour of fluoxetine and norfluoxetine using HPLC with respect to mobile phase composition, pH, flow rate and the amoun t of the native beta-cyclodextrin (beta-CD) or the beta-hydroxypropyl- cyclodextrin (HP-beta-CD), added to the mobile phase. Further, it was of interest to evaluate the effectiveness of beta-cD and HP-beta-CD to enhance fluorescence detection of these compounds. Another purpose of this study, was to calculate the formation constants of the inclusion complexes (K-f) of fluoxetine and norfluoxetine within the HP-beta-CD , in different mobile phase compositions. Based on these findings, the log K-f values of these two compounds referred to pure aqueous mobile phase (log K-fw), can be determined by extrapolation.