HPLC METHOD FOR THE DETERMINATION OF EDTA IN AN OPHTHALMIC CLEANSER

Authors
TRAN G CHEN C MILLER RB
Citation
G. Tran et al., HPLC METHOD FOR THE DETERMINATION OF EDTA IN AN OPHTHALMIC CLEANSER, Journal of liquid chromatography & related technologies, 19(9), 1996, pp. 1499-1508
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of liquid chromatography & related technologies → ACNP
ISSN journal
10826076
Volume
19
Issue
9
Year of publication
1996
Pages
1499 - 1508
Database
ISI
SICI code
1082-6076(1996)19:9<1499:HMFTDO>2.0.ZU;2-Y
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) proced ure using ultra-violet (UV) detection for the analysis of edetate diso dium (EDTA) via complexation with iron, in an ophthalmic cleanser, is reported. The method is selective, accurate, and reproducible. The pea k area versus EDTA concentration is linear over the range of 50-150% o f its label claim of 0.30 mg/mL, with a detection limit of 200 ng/mL. The mean absolute recovery of EDTA, using the described method, is 102 .7 +/- 0.4% (mean +/- SD, n = 10).