Mm. Laine et Ks. Jorgensen, STRAW COMPOST AND BIOREMEDIATED SOIL AS INOCULA FOR THE BIOREMEDIATION OF CHLOROPHENOL-CONTAMINATED SOIL, Applied and environmental microbiology, 62(5), 1996, pp. 1507-1513
We evaluated the use of straw compost and remediated soil as inocula f
or bioremediation of chlorophenol-contaminated soil, The in situ biotr
ansformation of pentachlorophenol (PCP) and mineralization of radiolab
eled [U-C-14]PCP by straw compost and remediated soil were studied und
er field-simulating conditions before and after 3 months of adaptation
with PCP in a percolator. After PCP adaptation, the straw compost min
eralized up to 56% of the [C-14]PCP, No partial dechlorination of PCP
was found. The native straw compost did not mineralize PCP, but partia
l dechlorination of PCP occurred (i) at pH 8 under near-thermophilic c
onditions (45 degrees C) and (ii) at pH 7 under aerobic and mesophilic
conditions, No biotransformation reactions occurred at room temperatu
re (25 degrees C) at pH 8, Enrichment in the percolator enhanced the m
ineralization rate of remediated soil to 56% compared with that of the
native remediated soil, which mineralized 24% of [C-14]PCP added, Tra
ce amounts of chloroanisoles as the only biotransformation products we
re detected in PCP-adapted remediated soil, Both inoculants studied he
re showed effective mineralization of PCP when they were adapted to PC
P in the percolator. No harmful side reactions, such as extensive meth
ylation, were observed.