IDENTIFICATION OF MULTIPLE PLASMIDS RELEASED FROM RECOMBINANT GENOMESOF HANSENULA-POLYMORPHA BY TRANSFORMATION OF ESCHERICHIA-COLI

Authors
GRAUPNER S WACKERNAGEL W
Citation
S. Graupner et W. Wackernagel, IDENTIFICATION OF MULTIPLE PLASMIDS RELEASED FROM RECOMBINANT GENOMESOF HANSENULA-POLYMORPHA BY TRANSFORMATION OF ESCHERICHIA-COLI, Applied and environmental microbiology, 62(5), 1996, pp. 1839-1841
Citations number
18
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
5
Year of publication
1996
Pages
1839 - 1841
Database
ISI
SICI code
0099-2240(1996)62:5<1839:IOMPRF>2.0.ZU;2-P
Abstract
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) str ains having chromosomal single or tandem multiple integrations of a pU C18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than t hat of the expression plasmid. Evidence that plasmid-like structures a re formed in H. polymorpha and that their formation is stimulated by D NA damage is presented in this study.