THE SEQUENCE OF A 24152 BP SEGMENT FROM THE LEFT ARM OF CHROMOSOME-XIV FROM SACCHAROMYCES-CEREVISIAE BETWEEN THE BN11 AND THE POL2 GENES

In the framework of the European Union programme for sequencing the ge nome of Saccharomyces cerevisiae we have determined the nucleotide seq uence of a region of 24 152 bp located on the left arm of chromosome X IV between the BNII and the POL2 genes. The sequence was obtained by d irected sequence analysis using a mixture of ExoIII and primer walking strategies. Subsequent analysis revealed 13 open reading frames (ORFs ) including four small ORFs completely internal to, or partly overlapp ing with, other ORFs. Five of these ORFs have been described previousl y (BNII, APL1, LYP1, PIK1, POL2) and thus 74.8% of the 24 152 bp were already present in the databases prior to this sequencing effort. Inte restingly, all 13 identified ORFs are characterized by a low codon ada ptation index (0.04-0.22). In addition, this region of chromosome XIV shows an unusually high gene density with about 88% of coding DNA. Thi s amounts to one gene per 2177'op, which is significantly above the av erage gene length (about 1500 bp). For eight ORFs considerable homolog ies to 'Expressed Sequence Tags' derived from human cDNAs located in t he XREF database could be identified. The complete nucleotide sequence of the 24 152 bp segment has been deposited in the EMBL data library under the Accession Number X92494.