ATP-dependent transport of Na-22(+) into liposomes reconstituted from
plasma membrane proteins of Heterosigma akashiwo was examined. The app
arent K-m values for transport of Na+ were 400 mu M for ATP and 7 mM f
or Na+. ATP-dependent transport of Na-22(+) was not inhibited by a pro
tonophore or a membrane-permeable cation but was inhibited by an inhib
itor of P-type ATPases.