RATE-LIMITING STEPS OF TYROSINASE-MODIFIED ELECTRODES FOR THE DETECTION OF CATECHOL

The response currents obtained for tyrosinase-modified Teflon/graphite , carbon paste, and solid graphite electrodes in the presence of catec hol are analyzed primarily using rotating disk electrode experiments. The rate-limiting steps, such as the electrochemical reduction of o-qu inones and the enzymatic reduction of oxygen as well as the enzymatic oxidation of catechol, are theoretically considered and experimentally demonstrated for the different electrode configurations.