GLYCOSIDASE-CATALYZED SYNTHESIS OF OLIGOSACCHARIDES - A 2-STEP SYNTHESIS OF THE CORE TRISACCHARIDE OF N-LINKED GLYCOPROTEINS USING THE BETA-N-ACETYLHEXOSAMINIDASE AND THE BETA-MANNOSIDASE FROM ASPERGILLUS-ORYZAE

Authors
SINGH S SCIGELOVA M CROUT DHG
Citation
S. Singh et al., GLYCOSIDASE-CATALYZED SYNTHESIS OF OLIGOSACCHARIDES - A 2-STEP SYNTHESIS OF THE CORE TRISACCHARIDE OF N-LINKED GLYCOPROTEINS USING THE BETA-N-ACETYLHEXOSAMINIDASE AND THE BETA-MANNOSIDASE FROM ASPERGILLUS-ORYZAE, Chemical communications, (8), 1996, pp. 993-994
Citations number
5
Categorie Soggetti
Chemistry
Journal title
Chemical communications → ACNP
ISSN journal
13597345
Issue
8
Year of publication
1996
Pages
993 - 994
Database
ISI
SICI code
1359-7345(1996):8<993:GSOO-A>2.0.ZU;2-P
Abstract
Using a partially purified beta-mannosidase from Aspergillus oryzae, a beta-mannosyl unit is transferred from p-nitrophenyl beta-D-mannopyra noside 5 to di-N-acetylchitobiose 4 to give the core trisaccharide ta- D-Manp-(1-->4)-beta-D-GlcpNAc-(1-->4)-D-GlcpNAc 6 of the N-linked glyc oproteins.