RAPID ANALYSIS OF AMPHETAMINES IN BLOOD USING HEAD SPACE-SOLID PHASE MICROEXTRACTION AND SELECTED-ION MONITORING

A simple and rapid method for analysis of methamphetamine (MA) and amp hetamine (AP) in blood was developed using head space-solid phase micr oextraction (HS-SPME) and gas chromatography-mass spectrometry/electro n impact ionization-selected ion monitoring (GC-MS/EI-SIM). A vial con taining a blood sample, sodium hydroxide, and pentadeuterated methamph etamine as an internal standard, was heated at 80 degrees C for 20 min . The extraction fiber of the SPME was exposed for 5 min in the head s pace of the vial. First, heptafluorobutyric anhydride solution was inj ected into the injection port of the GC-MS to make heptafluorobutyrami de (HFB) derivatives of amphetamines, and compounds absorbed on the fi ber were detached by exposing the fiber in the injection port. Straigh t calibration curves of MA and AP were obtained from 0.01 to 2 mu g/g in blood, respectively. No interfering substances were found, and the time for analysis was 30 min for one sample.