A model of an oximeter-based two-substrate enzyme electrode is present
ed. The simplifications of the general model lead to a solution to dif
ferential equations describing the influence of the rate of the enzyme
reaction in combination with the diffusion phenomena on measurable ox
ygen flux. Both transient phase and steady state conditions for experi
mental data can be considered within the frames of the model and provi
de possibilities for the calibration of the biosensor. The optimal par
ameters for the biosensor calibration can be used in practical design
of enzyme electrodes.