Jm. Li et Ac. Sartorelli, GRANULOCYTE-COLONY-STIMULATING FACTOR-RECEPTOR MEDIATED CELL-GROWTH AND DIFFERENTIATION, Oncology research, 7(12), 1995, pp. 591-601
Granulocyte colony-stimulating factor (G-CSF) can elicit different bio
logical responses through interaction with its receptor (G-CSFR). A se
ries of G-CSFR expressing clones were established from WEHI-3B D+ myel
omonocytic leukemia cells and interleukin-3 (IL-3) dependent Ba/F3 pre
B cells by transduction of a G-CSFR cDNA. The overexpression of the G-
CSFR from the expression vector promoted differentiation in WEHI-3B D cells exposed to G-CSF while, in contrast, the cytokine supported gro
wth in Ba/F3 cells transfected with the expression plasmid in the abse
nce of IL-3. Western blotting, immunoprecipitation and cell surface la
beling with Na[I-125] followed by immunoprecipitation with an anti-G-C
SFR antibody revealed different patterns of detected proteins in these
two cell lines, implying that different forms of the G-CSFR were gene
rated which produced distinct biological responses and/or that differe
nt cell-type specific signal transducers were involved in the producti
on of cellular differentiation or growth.