A. Yagi et al., GROWTH OF CULTURED HUMAN BRONCHIOGENIC EPITHELIOID CCD-14 BR CELLS AND DERMAL FIBROBLASTS, NB1 RGB TREATED WITH GINSENG TETRAPEPTIDE AND ITS ISOMER, Planta medica, 62(2), 1996, pp. 115-118
The configurations of the component amino acids in ginseng tetrapeptid
e 1 isolated from Panax ginseng were determined by HPLC with an optica
l resolution column and the structure of 1 was established to be H-L-V
al-gamma-D-Glu-D-Arg-Glu-OH. Synthesis of the ginseng tetrapeptide, 1,
and of the configuration and conjugation isomers, H-L-Val-gamma-L-Glu
-L-Arg-Gly-OH (2), H-L-Val-D-Glu-D-Arg-Gly-OH (3), and H-L-Val-L-Glu-L
-Arg-Gly-OH (4) was carried out by a solid-phase method using the Fmoc
strategy. The effects of 1-4 on the proliferation of baby hamster kid
ney (BHK)-21 cells, normal female bronchiogenic epithelioid (CCD-14 Br
) cells, and normal human epidermal fibroblast (NB1 RGB) were examined
, Only 1 showed 32 and 23% enhancement of BHK-21 and human CCD-14 Br c
ells growth, respectively, at a concentration of 13.6 mu M and 41% enh
ancement of NB1 RGB cells growth at a concentration of 32 mu M under t
he conditions employed. It was shown that both the configuration of th
e component amino acids and the peptide conjugation at a gamma-positio
n of D-Glu in 1 are important for proliferation of the cells. Compound
1 exerted a prominent effect on cell stimulation and growth rate with
out any morphological change and showed no cytotoxicity.